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(A) Schematic representation of an OrganoPlate® 3-lane tissue chip formed by two channels for cell seeding and a middle channel for gel loading. (B) Brightfield image and (C) confocal projection of HUVECs (red) seeded on top channel and HDLECs (green) seeded in bottom channel. Images taken after 24 h of culture under flow conditions. (D) Representative confocal max projection of HDLECs cultured in flow for 14 days in OrganoPlate® and treated for 24 h with DENV-2 NS1 (NS1) or control medium (untreated=Unt). Nuclei stained with <t>DAPI.</t> Merged images shows VE-cadherin (green) and F-actin (red) and DAPI (blue). Scale bar = 30 µm.
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(A) Schematic representation of an OrganoPlate® 3-lane tissue chip formed by two channels for cell seeding and a middle channel for gel loading. (B) Brightfield image and (C) confocal projection of HUVECs (red) seeded on top channel and HDLECs (green) seeded in bottom channel. Images taken after 24 h of culture under flow conditions. (D) Representative confocal max projection of HDLECs cultured in flow for 14 days in OrganoPlate® and treated for 24 h with DENV-2 NS1 (NS1) or control medium (untreated=Unt). Nuclei stained with <t>DAPI.</t> Merged images shows VE-cadherin (green) and F-actin (red) and DAPI (blue). Scale bar = 30 µm.
Dapi Nuclear Stain, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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(A) Schematic representation of an OrganoPlate® 3-lane tissue chip formed by two channels for cell seeding and a middle channel for gel loading. (B) Brightfield image and (C) confocal projection of HUVECs (red) seeded on top channel and HDLECs (green) seeded in bottom channel. Images taken after 24 h of culture under flow conditions. (D) Representative confocal max projection of HDLECs cultured in flow for 14 days in OrganoPlate® and treated for 24 h with DENV-2 NS1 (NS1) or control medium (untreated=Unt). Nuclei stained with <t>DAPI.</t> Merged images shows VE-cadherin (green) and F-actin (red) and DAPI (blue). Scale bar = 30 µm.
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dapi nuclear stain  (Cell Signaling Technology Inc)
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(A) Schematic representation of an OrganoPlate® 3-lane tissue chip formed by two channels for cell seeding and a middle channel for gel loading. (B) Brightfield image and (C) confocal projection of HUVECs (red) seeded on top channel and HDLECs (green) seeded in bottom channel. Images taken after 24 h of culture under flow conditions. (D) Representative confocal max projection of HDLECs cultured in flow for 14 days in OrganoPlate® and treated for 24 h with DENV-2 NS1 (NS1) or control medium (untreated=Unt). Nuclei stained with <t>DAPI.</t> Merged images shows VE-cadherin (green) and F-actin (red) and DAPI (blue). Scale bar = 30 µm.
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dapi  (Bio-Rad)
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(A) Schematic representation of an OrganoPlate® 3-lane tissue chip formed by two channels for cell seeding and a middle channel for gel loading. (B) Brightfield image and (C) confocal projection of HUVECs (red) seeded on top channel and HDLECs (green) seeded in bottom channel. Images taken after 24 h of culture under flow conditions. (D) Representative confocal max projection of HDLECs cultured in flow for 14 days in OrganoPlate® and treated for 24 h with DENV-2 NS1 (NS1) or control medium (untreated=Unt). Nuclei stained with <t>DAPI.</t> Merged images shows VE-cadherin (green) and F-actin (red) and DAPI (blue). Scale bar = 30 µm.
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(A) Schematic representation of an OrganoPlate® 3-lane tissue chip formed by two channels for cell seeding and a middle channel for gel loading. (B) Brightfield image and (C) confocal projection of HUVECs (red) seeded on top channel and HDLECs (green) seeded in bottom channel. Images taken after 24 h of culture under flow conditions. (D) Representative confocal max projection of HDLECs cultured in flow for 14 days in OrganoPlate® and treated for 24 h with DENV-2 NS1 (NS1) or control medium (untreated=Unt). Nuclei stained with DAPI. Merged images shows VE-cadherin (green) and F-actin (red) and DAPI (blue). Scale bar = 30 µm.

Journal: bioRxiv

Article Title: Lymphatic vessel dysfunction contributes to severe dengue pathogenesis

doi: 10.64898/2026.03.27.714698

Figure Lengend Snippet: (A) Schematic representation of an OrganoPlate® 3-lane tissue chip formed by two channels for cell seeding and a middle channel for gel loading. (B) Brightfield image and (C) confocal projection of HUVECs (red) seeded on top channel and HDLECs (green) seeded in bottom channel. Images taken after 24 h of culture under flow conditions. (D) Representative confocal max projection of HDLECs cultured in flow for 14 days in OrganoPlate® and treated for 24 h with DENV-2 NS1 (NS1) or control medium (untreated=Unt). Nuclei stained with DAPI. Merged images shows VE-cadherin (green) and F-actin (red) and DAPI (blue). Scale bar = 30 µm.

Article Snippet: The slides were then incubated with the nuclear stain DAPI (cat# D9542, Merck) at 1:1000 dilution for 10 min and mounted onto glass slides using mounting medium (InvitrogenTM Fluoromount-GTM Mounting Medium-00495802).

Techniques: Cell Culture, Control, Staining